Title: Improved detection of species through environmental DNA: methods and example with the American Bullfrog

Abstract : The ability to detect e.g. secretive species or species at a low density greatly determines the success of inventories, eventually eradication in the case of invasive species, and decreases both the inventory and control action costs and the impact on ecosystems. In this talk, we will present the method called environmental DNA (eDNA) which was recently used in order to detect species through extracellular DNA present in environmental samples, coming from cell lysis or living organism excretion or secretion. We will provide some examples for species detection using eDNA from freshwater and soil samples. We will also focuse on comparing effectiveness of traditional field methods, based on auditory and visual encounter surveys, with an eDNA survey for detecting the invasive American bullfrog (Lithobates catesbeianus).